The significance of biotic and abiotic factors in ecosystems lies in their interconnected roles in sustaining life. Biotic factors include all living organisms, such as plants, animals, and microorganisms, while abiotic factors encompass non-living elements like water, soil, and climate. Together, these factors create the necessary conditions for survival and are vital in determining the structure and function of ecosystems. Changes in either factor can significantly impact biodiversity and ecosystem health.