The primary role of autotrophs in the food chain is to produce organic matter from inorganic substances through processes such as photosynthesis and chemosynthesis. Autotrophs, including plants, algae, and some bacteria, harness energy from sunlight or chemical reactions to create food, serving as the foundational energy source for all higher trophic levels. By converting solar or chemical energy into biomass, autotrophs support a diverse array of organisms, ensuring ecosystem stability and resilience throughout the food web.