The significant document that announced the end of dispute between Spain and Portugal in the Americas was the Treaty of Tordesillas, signed in 1494. This treaty was brokered by Pope Alexander VI to resolve conflicts arising from both nations' claims to territories in the New World following Christopher Columbus's voyages. The treaty established a meridian, granting Spain rights to territories west of the line and Portugal rights to lands east of it. Although the treaty aimed to prevent conflict between the two nations, its imprecise demarcations led to disputes and challenges to its authority, shaping colonial competition in the Americas.