Prolonged sitting impacts postural alignment by leading to muscle imbalances and tightness, particularly in the hip flexors and lower back, while weakening core and gluteal muscles. These changes can result in poor posture, contributing to discomfort and increased risk of injuries. Sitting for extended periods can lead to conditions like sciatica and increased spinal degeneration. To mitigate these effects, it's crucial to integrate regular breaks, stretching, and strengthening exercises that promote proper alignment and support musculoskeletal health.