The major global conflict that affected the life and work of Anne Frank was World War II. During this period, the Nazi regime implemented oppressive policies against Jews, leading to the Frank family's decision to go into hiding in Amsterdam. Anne's experiences during the war were documented in her diary, which revealed the harsh realities of living in fear and isolation. The impact of the war on her life and the eventual discovery of the hiding place tragically underscored the human cost of war and persecution, making her story a powerful symbol of resilience and loss.